Investigation of Compatibility Between Serological Tests Used in Laboratory Diagnosis of Brucellosis

Aim: In the diagnosis of brucellosis, production microorganisms in blood or bone marrow culture is considered gold standard. But it not always possible to produce microorganism. For this reason, serological tests are used diagnose brucellosis. Rose Bengal test (RB), Standard Tube Agglutination (STA), Coombs Test (CT) and 2-Mercaptoethanol (2-ME) common methods. Immunocapture-agglutination (ICA) Brucella Gel (BCGT) that can detect blocking antibodies along with total antibodies. The aim our study was determine whether there compatibility between ICA BCGT tests.Material Methods: Serum samples were taken from patients a preliminary RBT testing performed primarily on all samples. Forty positive 40 negative sera included study. All serum studied by STA, CT, 2-ME, determined using kappa (κ) coefficient Cohen analysis method.Results: 28 detected as STA 2-ME 30 them CT. results found be Kappa compliance (Kappa 1.0 <0.001) excellent. Compliance good 0.7 p< 0.001). CT showed very level 0.8 p<0.001). There 2ME test, (kappa 0.001), also 1.0, p<0.001).Conclusion: According these results, use tests, both number will reduced time receive reduced. further studies needed sensitivity specificity based results. after that, we believe diagnostic test.